Tag

Part:BBa_K237008:Design

Designed by: Marc T. K. Nielsen   Group: iGEM09_SDU-Denmark   (2009-10-21)

OmpA leader sequence - Targeting to periplasm, cleaved during transport


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We chose this localisation signal for use with our project BBa_K237003. Sadly we didn't finish this part, so we can't verify that it works, however numerous applications of it has been described in the litterature (see reference), so it is very fair to expect it to. 

***WARNING*** This part should not be used with an assembly standard that introduces scars into the sequence due to the possibility of frameshifts.

Source

http://www.pharmcast.com/Patents/Yr2003/May2003/052003/6566108_Cytochrome052003.htm

References

Recombinant protein secretion in Escherichia coli. F J M Mergulhão, D K Summers, and G A Monteiro Biotechnology advances 23 (3), 177-202 (May 2005) info:pmid/15763404 | info:doi/10.1016/j.biotechadv.2004.11.003